Técnicas moleculares como: secuenciación de DNA, Patrones de Restricción Polimórfica y estudios filogenéticos basados en la porción Interna Transcrita (ITS) del DNA ribosomal, fueron utilizadas en este estudio para la identificación de los especimenes.

Collybia sensu lato es un grupo de hongos de gran importancia, puesto que con el pasar de los años ha estado involucrado en controversias taxonómicas. Ellos son saprófitos que pueden producir agentes antimicrobianos y enzimas de importancia industrial. Este grupo de hongos perteneciente a la división Basidiomycota, Orden Agaricales. Un censo de dieciocho meses fue realizado en la parte central y oeste de Puerto Rico con el propósito de colectar hongos del género Collybia sensu lato (s.l). Finally, phylogenetics studies based on ITS region of rDNA shows that Marasmius rhyssophylus is more related to the genus Tricholomopsis than to Marasmius, suggesting a new combination: Tricholomopsis rhyssophylus. in Puerto Rico is restricted to the genus Gymnopus. Also the microscopic and molecular studies suggest that Collybia s.l. A specific restriction digestion patterns based in 18S of rDNA, using HaeIII endonuclease, shows a possible biological genetic marker for the genus Gymnopus. Five of the six Collybia-like fungus tested produced a substance that inhibited the growth of gram-positive and gram-negative bacteria. collected were tested to determine the production of microbial inhibitory substances. Fourteen Collybioid fungus and related genera were identified in this study eleven Gymnopus, two Crinipellis and one Marasmius. Light (phase contrast) and Scanning Electron Microscopy techniques were used to morphologically describe the Collybia s.l.

Molecular approaches such as: DNA sequencing, Restriction Fragment Length Polymorphism and phylogenetics studies based in Internal Transcribed Spacer of rDNA, were used in this study to identify the specimens collected. Collybia sensu lato is an important group of fungi because over the years have been involved in many taxonomical discrepancies. They are saprophytes and can produce antimicrobial agents and enzymes with industrial significance. The genus Collybia is in the Basidiomycota division, order Agaricales. An eighteen month survey at the central and western regions of Puerto Rico was done to monitor Collybia sensu lato (s.l.).

Microscopia de contraste de fases